Dr. R.J.M.M. Thoolen

Managing Director / Dutch Board Certified Toxicologic Pathologist (PhD, CRP/TP)

Bob is the founder and Managing Director of GPS BV. He has over 30 years education and/or experience in pathology. Training in human- and animal pathology gives him a broad perspective of the science of pathology. First working at the Central Veterinary Institute and later at Solvay Pharmaceuticals B.V. as Toxicologic Pathologist, is a sound basis of Toxicologic Pathology as performed up to now under GLP regulations at GPS BV. In his free time he like to fitness, swimming and play golf as much as possible.

Working period at GPS: 17 years

Education
Professional Experience
Publications
Memberships
Studies track record

Education

2007 – 2012
PhD project on non-proliferative and proliferative liver lesions (University Medical Center Utrecht (UMCU), Department of Pathology, Division of Laboratories and Pharmacy).
1997 – 1999
Study Medicine (1-3rd year) at the Faculty of Medicine, Royal University of Utrecht.
1996
Board Certified Toxicologic Pathologist, by the Committee set up jointly by the Pathological Society of The Netherlands and The Royal Netherlands Veterinary Association.
1993
Qualified Laboratory Animal Pathologist, by the Committee set up jointly by the Pathological Society of The Netherlands and the Royal Netherlands Veterinary Association.
1989 – 1993
Postgraduate training for Laboratory Animal Pathologist.
1989 – 1991
Postgraduate course in Microscopical Pathology.
1988 – 1989
Training program “Veterinary Pathology” (theory and practical training for Veterinary students. Studying Pathology of Laboratory animals.
1988
Masters of Science in Medical Biology (B5*).
1987
Training program “Laboratory Animal Science” (Art. 9).
1985 – 1986
Examinations and practical training in General and Special Pathology of Medicine at the Academic Hospital Utrecht, Department of Pathology.
1981 – 1988
University of Utrecht 1985 Bachelor of Science in Medical Biology.

Professional Experience and Training

Total years of training and experience: 28 years
Formal examinations and practical training in General and Special Pathology of Medicine at the Academic Hospital Utrecht, Department of Pathology for one year (1985-1986) – Prof. Dr. J.A.M. van Unnik, Dr. C. Gielis.
Training program “Veterinary Pathology” (theory and practical training for Veterinary students. Studying Pathology of laboratory animals for one year (1988-1989) – Prof. dr. P. Zwart.
Pathologist and pathology training at the Central Veterinary Institute at Lelystad (1989-1991) – Prof. dr. J.E. van Dijk.
Formal post-graduate course in Microscopical Pathology for two years at the Veterinary Faculty, Department of Veterinary Pathology (1989-1991) – Prof. dr. J. Mouwen.
Formal post-graduate programme for Laboratory Animal Pathologist (1989-1993) – Prof. dr. J.E. van Dijk (Royal University Utrecht) and R. Dawes (Solvay).
Formal courses for certification as Toxicologic Pathologist (1993-1996) – Prof. dr. J.E. van Dijk and Prof. dr. J. Vos.
Formal examinations and practical training in General and Special Pathology of Medicine at the Academic Hospital Utrecht, Department of Pathology during study of Medicine (1997) – Prof. dr. van den Tweel.
Pathologist at Solvay in department of Toxicology/Drug Safety/Preclinical Drug validation Unit (1992-2004).
Toxicologic Pathologist/Managing Director of Global Pathology Support (2004-now)
Set up Gobal INHAND Liver Nomenclature document as BSTP representative (first author amongst representatives of the STP, ESTP, and GSTP respectively (2007-2010.

Publications

Major contributions to approved internal reports; FDA submissions, IDB, NDAs, Expert reports and Appraisals of Solvay Pharmaceuticals B.V. – Titles not presented.
Bob Thoolen. The mouse renal system – A summary of histopathological findings. Dept. Veterinary Pathology, Div. Pathology of Laboratory and special animal diseases, November 1988; 16 pp.
Bob Thoolen. Rat – Histopathology of the renal system – A summary of histopathological findings. Dept. Veterinary Pathology, Div. Pathology of Laboratory and special animal diseases, November 1988; 18 pp.
BrdUrd labeling of S-Phase Cells in Testes and Small Intestine of Mice, Using Microwave Irradiation for Immunogold-Silver Staining: An Immunocytochemical Study. The J. Histochem. & Cytochem. 1990; 38(2):267-273.
Bob Thoolen. Malignant Fibrous Histiocytoma – a histogenetic study – The J. Comp. Pathol. 1990 (Submitted).
R.J.M.M. Thoolen, J.H. Vos, J.S. van der Linde-Sipman, R.A. de Weger, J.A.M. van Unnik, W. Misdorp and J.E. van Dijk. Malignant Fibrous Histiocytomas in Dogs and Cats – An Immunohistochemical Study – Res. Vet Sci. 1992; 53:198-204.
Bob Thoolen, Henk Koster, Anton van Kolfschoten & Martin de Haan. Gastric neuroendocrine tumours in a two-year oncogenicity study with CD-1 mice. Toxicol Pathol 2002; 30 (3):322-327.
Ben A.R. Lina, Ruud A. Woutersen, Joost A. Bruijntjes, Jan van Benthem, Jolanda A.H. van den Berg, Johan Monbaliu, Bob J.M.M. Thoolen, Rudolf B. Beems and Coen F. van Kreijl. Evaluation of the Xpa-Deficient Transgenic Mouse Model for Short-Term Carcinogenicity Testing: 9-Month Studies with Haloperidol, Reserpine, Phenacetin, and D-Mannitol. Toxicol Pathol 2004; 32 (2):192-201.
Baras B, Stittelaar KJ, Simon JH, Thoolen RJMM, Mossman SP, et al. (2008) Cross-Protection against Lethal H5N1 Challenge in Ferrets with an Adjuvanted Pandemic Influenza Vaccine. PLoS ONE 3(1): e1401. doi:10.1371/journal.pone.0001401.
Bob Thoolen, Robert R. Maronpot, Takanori Harada, Abraham Nyska, Colin Rousseaux, Thomas Nolte, David E. Malarkey, Wolfgang Kaufmann, Karin Küttler, Ulrich Deschl, Dai Nakae, Richard Gregson, Michael P. Vinlove, Amy E. Brix, Bhanu Singh, Fiorella Belpoggi1, Jerrold M. Ward.Nomenclature and Diagnostic Criteria for Hepatobiliary Lesions in Rats and Mice (INHAND) Toxicol Pathol 2010; 38:5S-81S.
Jerald M. Ward and Bob Thoolen. Grading of Lesions. Toxicol Pathol 2011; 39:745-746.
Bob Thoolen, Fiebo J.W. ten Kate, Paul J. van Diest, David E. Malarkey, Susan A. Elmore and Robert R. Maronpot. Comparative Histomorphological Review of Rat and Human Hepatocellular Proliferative Lesions. J Toxicol Pathol 2012;23:23-36.
Bob Thoolen, Fiebo J.W. ten Kate, Domenico Castigliego, Paul J. van Diest, David E. Malarkey, Susan A. Elmore and Robert R. Maronpot. Comparative Immunohistochemical Investigation of Rat and Human Hepatocellular Carcinomas. J Toxicol Pathol 2012 (Accepted).
Bob Thoolen, Fiebo J.W. ten Kate, Domenico Castigliego, Paul J. van Diest, David E. Malarkey, Susan A. Elmore and Robert R. Maronpot. Immunohistochemical Research on Human and Rat Hepatocellular Proliferative Lesions. J Histochem & Cytochem (Manuscript in preparation).

Professional Memberships

1992 - current
Member of The Netherlands Society of Toxicology.
1992 – 1993
Member of the Charles Lewis Foundation.
1993 – current
Elected Member of The Pathological Society of the Netherlands.
1993 – current
Elected Member of The Society of Toxicologic Pathologists.
2004 – 2010
Member of the Board of the European Society of Toxicologic Pathology (ESTP).
2007
Member of the British Society of Toxicologic Pathology.
2005 – 2010
Member of the Educational Board of the Netherlands Society for Pathology.

Studies Track Record (1992-2021)

GLP-studies
TYPE OF STUDY
SOLVAY (1992 - 2004)
GLOBAL PATHOLOGY SUPPORT B.V. (2004 - 2020)
Acute dog/rat/mouse/rabbit
+/- 800
+/- 95
Subchronic rat studies
+/- 200
+/- 180
Subchronic dog studies
+/- 100
+/- 80
Subchronic monkey studies
+/- 50
+/- 65
Carcinogenicity studies
+/- 10
+/- 4
Reproduction/Developmental/ Toxicity studies (OECD 421,422 & 443)
+/- 10
+/- 30
Peer review (sub-)chronic studies-
+/- 100
+/- 35
Peer review carcinogenicity studies
+/- 10-20
+/- 6

Several (n=10-20) readings of studies with other species like: guinea pigs, ferret, hamster (chronic and carcinogenicity studies), minipigs (subchronic and chronic studies) were performed.
The application routes of all studies contained : oral (gavage, diet), intravenous, inhalation, subcutaneous, transdermal, dermal, intra-dermal, infusion, intra-peritoneal and sublingual administration routes.
Histopathology of Reproduction/Developmental/ Toxicity studies involving study of reproduction organs in detail (OECD 421 and 422). Specialised reproduction and neuropathology investigations (qualitative and quantitative) involving specific brain localizations under OECD guidance (OECD 443).

ALL studies performed by Global Pathology Support B.V. were under GLP and approved for submission.

Pre-clinical pathology evaluation of drugs, vaccines and chemicals in toxicology studies for safety assessment. Global Pathology Support B.V. deals with preclinical safety – toxicologic pathology - for the (bio-)pharmaceutical and chemical industry.

Company Info

Churchillplein 6D
2517 JW Den Haag
the Netherlands

+31 (0)70 314 24 04
+31 (0)6 461 496 05

bob.thoolen@gpstoxpath.com

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